Find spots using information from two channels

spots.combined(
  red,
  green,
  mask,
  size = NULL,
  voxelsize = NULL,
  thresh.offset = 0.1,
  window = c(5, 5),
  min.sum.intensity = 2,
  max.distance = 0.5,
  use.brightest = FALSE,
  max.spots = NA,
  full.voxel = FALSE
)

Arguments

red: image
green: image
mask: image mask
size: size of img in microns
voxelsize: size of voxel in microns
thresh.offset: Thresh offset used in EBImage::thresh()
window: Half width and height of the moving rectangular window.
min.sum.intensity: spots smaller than min.sum.intensity are ignored
max.distance: use only spots with distance to other color spot smaller than max.distance
use.brightest: Logical; use only brightest in max.distance?
max.spots: maximum of spots (per channel), only when use brightest=TRUE
full.voxel: Logical; output contains full voxel instead of rgb intensities

Value

RGB image with spots will be written to output folder