Detects spots for one file
Usage
find.spots.file(
file,
dir,
color,
thresh = NULL,
thresh.auto = FALSE,
thresh.quantile = 0.9,
filter = NULL,
cores = 1
)Arguments
- file
file
- dir
directory for results
- color
which color, images have to be in folder with color name
- thresh
threshold
- thresh.auto
Logical. Find threshold automatically?
- thresh.quantile
numeric. use simple
- filter
2d-filter to use before spot detection
- cores
number of cores to use in parallel (with parallel package only)